https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34323 Rhodopseudomonas palustris C1 > R. palustris AB3 > Rubrivivax benzoatilyticus C31 > R. palustris L28 which corresponded to the presence of As‐resistance genes in these bacteria. The strain C1 showed all As‐marker genes; arsC, arsM, aioA, and acr3, while aioA was not detected in strain AB3. Strains C31 and L28 had only Arsenite‐transporter gene, acr3. Translation of all these detected gene sequences of strain C1 to amino acid sequences showed that these proteins have vicinal cysteine; Cys126, Cys105, and Cys178 of Acr3, ArsC, AioA, respectively. Tertiary structure of proteins Acr3, ArsC, AioA, and ArsM showed strain C1 exhibits the high activities of arsenite oxidase and arsenate reductase enzymes that are encoded by aioA and arsC genes, respectively. Moreover, strain C1 with arsM gene produced volatile‐methylated As‐compounds; monomethylarsonic acid (MMA), dimethylarsenic acid (DMA), and arsenobetaine (AsB) in the presence of either As(III) or As(V). In conclusion, the strain C1 has great potential for its application in bioremediation of As‐contaminated sites.]]> Wed 27 Feb 2019 12:32:39 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29583  L28 > C1. Strains AB3, C31, and L28 accumulated higher levels of intracellular vitamin B12 than strain C1. However, only strain C1 produced SAM and showed As biovolatilization activity. When strain C1 was incubated under microaerobic light conditions, it produced arsenobetaine [(AsB(V)] in medium with As(III) and monomethylarsonic acid [MMA(V)] in medium with As(V), which were found in the culture supernatants. Volatile methylated As compounds, such as dimethylarsenic acid [DMA(V)] and MMA(V)], were also detected in strain C1 during 30 days of incubation with As(III) and As(V). Strains AB3, C1, and L28 were identified as Rhodopseudomonas palustris and strain C31 as Rubrivivax benzoatilyticus. The resistance of these strains to As [As(III) and As(V)] based on minimum inhibition concentration values under aerobic dark and microaerobic light conditions, respectively, was in the order of C1 > AB3 > C31 > L28. The overall results demonstrate that all four selected strains of PNSB have a great potential for future application in the remediation of As-contaminated areas although R. palustris C1 was the most effective candidate.]]> Tue 31 Jul 2018 16:28:15 AEST ]]>